TISSUE CULTURE STUDIES ON SOLANUM TORVUM (SWARTZ)

Solanum torvum (Swartz) plants were tested for callus induction callus-mediated regeneration direct regeneration micropropagation somatic embryogenesis and protoplast isolation. Below are the work''s principal results. To create a callusing protocol for hypocotyl cotyledon and leaf explants on a growth regulator-containing medium. Standardize callus-mediated regeneration on varied growth regulator concentrations and combinations. To examine the influence of growth ingredient concentrations and combinations on in vitro plantlet regeneration in leaf and cotyledon explants. Shoo the tip and nodal explants from 30-day-old seedlings induced numerous shoots. Solanum torvum leaf explants somatic embryogenesis and plantlet development. Explants from 30-day-old seedlings planted in glass bottles generated more numerous shoots (8.0/explant) than those grown in glass tubes. In vitro shoots rooted in MS medium with 2.0 mg/L IAA/IBA. After greenhouse hardening 95% of tissue-culture plantlets survived. Rooted shoots survived (92%) greenhouse hardening and matured (100%) in the field. Leaf explants isolate high amounts of protoplast.