Tissue Fixation and Processing

This comprehensive manual provides an in-depth exploration of tissue fixation processing and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation the book delves into the chemistry and classification of various fixatives including aldehydes oxidizing agents protein denaturants and cross-linking compounds highlighting their mechanisms applications and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation such as pH temperature penetration rate and osmolality. Detailed discussions cover both conventional and advanced methods like microwave fixation and the use of secondary fixatives for enhanced preservation. The book also provides extensive guidance on tissue processing stages including dehydration clearing impregnation and embedding using paraffin celloidin resins and ester wax. Special sections include techniques for photomicrography image analysis and tumor marker identification offering valuable insights for diagnostic and research applications.