TLC Analysis of Nail Polish Colour by Applying a Penetrative Dye

TLC is based on the classic chromatography principle where mixture components are separated between a fixes stationary phase and a liquid mobile phase by differential affinities between the two phases.Different compounds travel at different rates due to the differences in their attraction to the stationary phase and due to the differences in solubility in the solvent.Separation of compounds is based on the competition of the solute and the mobile phase for binding places on the stationary phase.