v-Myb proteins and their oncogenic potential

Master's Thesis from the year 2011 in the subject Biology - Micro- and Molecular Biology grade: 10 University of Münster (Institut für Biochemie) language: English abstract: The oncogene v-myb of the retroviruses AMV (avian myeloblastosis virus) and E26 (avian leukaemia virus) encodes a transcription factor (v-Myb) which is a truncated homolog of its cellular progenitor c-Myb. c-Myb plays an essential role in the development of haematopoietic cells and is known to be a regulator for many target genes. v-Myb AMV is responsible for the transformation of myelomonocytic cells and arrests them in an immature stage presumably by deregulating the expression of specific target genes. In addition to truncation of the coding region a number of amino acid (aa) substitutions are responsible for the high oncogenicity of v-Myb AMV. Due to the aa substitutions v-Myb AMV and v-Myb E26 differ in their target gene spectrum. Surprisingly the chicken mim-1 gene can be activated by v-Myb E26 and c-Myb but not by v-Myb AMV. Recently it was shown that two aa substitutions in a hydrophobic patch in the transactivation domain of v-Myb AMV are sufficient to disrupt its ability to stimulate the Myb-responsive enhancer in mim-1. This thesis focused on the consequences of these aa substitutions at the level of protein-protein interactions particularly investigating the hydrophobic regions of v-Myb AMV and v Myb E26. In this study a cytosolic variant of GRP78 GRP78va was confirmed to interact with both v-Myb proteins. It was shown that its interaction site is limited to a small region of v-Myb preceding the hydrophobic patch. Additionally it was shown that GRP78va also associated with other members of the Myb-family. Furthermore reporter gene experiments demonstrated a repressing effect of GRP78va on the transactivation potential of v-Myb E26.Two other proteins were tested for their interaction with the hydrophobic patch of v-Myb. Co-immunoprecipitation experiments confirmed that CC