Xylanases of Fungi from Extreme Habitats in Egypt

In this book the following methods and techniques are described and illustrated in details: 1- The methods for chemical analyses of different water mud and soil samples. 2- Isolation of mesophilic halotolerant/halophilic and thermotolerant/thermophilic fungi from air water mud and soil. 3- Extraction of xylan from different sources and its assay by UV-spectroscopy and HPLC. 4- Screening the potential of different fungi to produce xylanases. 5- Optimization of nutritional and environmental factors for the maximization of xylanases production. 6- Different techniques of xylanases production such as solid-state and submerged fermentation. 7- Determination of relative and specific activities as well as the total protein for carboxy methyl cellulase avicellase and xylanase enzymes. 8- Purification processes of xylanase enzyme such as: ammonium sulfate precipitation dialysis ion-exchange column chromatography and gel filtration column chromatography. 9- Characterization of xylanase enzyme.